ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of TaqMan? Real-time Reverse Transcription-Polymerase Chain Reaction for the Quantification of Feline Leukemia Virus Load)

ผู้เขียน:วิชญะ ทองตะโก, Therapol Sirinarumitr

สื่อสิ่งพิมพ์:pdf

Abstract

Feline leukemia virus (FeLV), a gamma retrovirus of the domestic cat, is not only of veterinary interest, but is also an important model for the study of pathogenesis of tumors and AIDS in many animals. After initial infection, disease progression has been associated with cellular and humoral immune response and FeLV proviral load. Moreover, an earlier study showed that not only the proviral load, but also the plasma viral loads are important parameters, which are associated with progression of the disease. In this study, the TaqMan? real-time reverse transcription-polymerase chain reaction (RT-PCR) was developed for the quantification of FeLV loads. The assay was developed to amplify a 131 base pairs conserved domain within the unique region (U3) of a long terminal repeat (LTR). The detection limit of this assay was 8.3 copies of RNA standard template or 4.15 viruses per reaction that is equivalent to 1,778 viruses per ml of plasma or serum.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 5, Dec 09 - Dec 09, Page 153 - 158 |  PDF |  Page